The titer of agglutinins vary with individuals and also depends upon the varieties f test, temperature and duration before .the test is conducted. An attempt was made to clearify the variations in serum agglutinin titer in the stored blood. The results obtained are as follows:
The sera . used in this study were diluted by the double and gradual dilution method to observe the variation of anti-A type and anti-B type agglutinin titers in the stored blood. The titer was first determined´ on the day the blood was taken, and thereafter, four consecutive determinations were made for the same sample at the interval of one week. In the light of individual differences in the agglutinogen titer, two sand same persons having blood type A and type B .were selected, and their erythrocytes were used throughout the tests. :
Nineteen stored blood samples were tested, and a little changes in general, were noticed up to 3 weeks. Only 2 cases of type A showed a slight diminution of titer. Slight diminution titers were noticed in the majority of cases after the 4th week. Therefore, the tendency of diminution of agglutinin titers in the stored blood becomes more remarkable as the time of storage goes on.. After the 5th week of storage of the blood, the results of the test are hardly to be interpreted, due mainly to hemolysis.
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